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ZWILCH Polyclonal antibody

ZWILCH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14281-1-AP

Print datasheet

Synonyms

Protein zwilch homolog, KNTC1AP, hZwilch

验证数据展示

  • IHC staining of human colon cancer using 14281-1-AP

    IHC staining of human colon cancer using 14281-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14281-1-AP (ZWILCH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of PC-3 using 14281-1-AP

    WB analysis of PC-3 using 14281-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14281-1-AP (ZWILCH Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

14281-1-AP targets ZWILCH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ZWILCH fusion protein Ag5454 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Zwilch, kinetochore associated, homolog (Drosophila)
Synonyms Protein zwilch homolog, KNTC1AP, hZwilch
Calculated Molecular Weight 67 kDa
Observed Molecular Weight ~60 kDa
GenBank Accession NumberBC036900
Gene Symbol ZWILCH
Gene ID (NCBI) 55055
RRIDAB_2878040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZWILCH is a new component of the ZW10/ROD complex. The complex is a key player in the spindle checkpoint. ZWILCH has two isoforms with MW 67 kDa and 54 kDa.

实验方案

Product Specific Protocols
WB protocol for ZWILCH antibody 14281-1-APDownload protocol
IHC protocol for ZWILCH antibody 14281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan
View Article
humanIHC

Biomedicines

The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients

Authors - Małgorzata Blatkiewicz
View Article