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ZWINT Polyclonal antibody

ZWINT Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 12282-2-AP

Print datasheet

Synonyms

Zwint-1, ZWINT1, Zwint 1, ZW10-interacting protein 1, KNTC2AP

验证数据展示

  • WB analysis of HepG2 using 12282-2-AP

    WB analysis of HepG2 using 12282-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12282-2-AP (ZWINT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 12282-2-AP

    IF Staining of HepG2 using 12282-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZWINT antibody (12282-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • FC experiment of HeLa using 12282-2-AP

    FC experiment of HeLa using 12282-2-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZWINT (12282-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12282-2-AP targets ZWINT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ZWINT fusion protein Ag2933 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ZW10 interactor
Synonyms Zwint-1, ZWINT1, Zwint 1, ZW10-interacting protein 1, KNTC2AP
Calculated Molecular Weight 277 aa, 31 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC020979
Gene Symbol ZWINT
Gene ID (NCBI) 11130
RRIDAB_2221166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZWINT antibody 12282-2-APDownload protocol
IF protocol for ZWINT antibody 12282-2-APDownload protocol
FC protocol for ZWINT antibody 12282-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols