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验证数据展示

  • WB analysis of HepG2 using 12282-2-AP

    WB analysis of HepG2 using 12282-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12282-2-AP (ZWINT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 12282-2-AP

    IF Staining of HepG2 using 12282-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZWINT antibody (12282-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • FC experiment of HeLa using 12282-2-AP

    FC experiment of HeLa using 12282-2-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZWINT (12282-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ZWINT Polyclonal antibody

ZWINT Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 12282-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Zwint-1, ZWINT1, Zwint 1, ZW10-interacting protein 1, KNTC2AP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12282-2-AP targets ZWINT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 ZWINT fusion protein Ag2933 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ZW10 interactor
别名 Zwint-1, ZWINT1, Zwint 1, ZW10-interacting protein 1, KNTC2AP
计算分子量 277 aa, 31 kDa
观测分子量 35 kDa
GenBank蛋白编号BC020979
基因名称 ZWINT
Gene ID (NCBI) 11130
RRIDAB_2221166
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95229
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZWINT antibody 12282-2-APDownload protocol
IF protocol for ZWINT antibody 12282-2-APDownload protocol
FC protocol for ZWINT antibody 12282-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols