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验证数据展示

  • WB analysis of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

    WB analysis of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60254-1-Ig (Zyxin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

    IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZYX antibody included
  • IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

    IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZYX antibody included
  • IF Staining of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

    IF Staining of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60254-1-Ig (Zyxin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

Zyxin Monoclonal antibody, PBS Only

Zyxin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60254-1-PBS

产品说明书

CloneNo. 4D7E7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ESP 2, HED 2, ZYX, zyxin, Zyxin 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ZYX Ready-To-Use IHC Kit. ZYX primary antibody included.

产品信息

60254-1-PBS targets Zyxin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Zyxin fusion protein Ag0419 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zyxin
别名 ESP 2, HED 2, ZYX, zyxin, Zyxin 2
计算分子量 80 kDa
观测分子量 78 kDa
GenBank蛋白编号BC008743
基因名称 Zyxin
Gene ID (NCBI) 7791
RRIDAB_2881375
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15942
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.