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Zyxin Monoclonal antibody, PBS Only

Zyxin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D7E7

Cat no : 60254-1-PBS

Print datasheet

Synonyms

ESP 2, HED 2, ZYX, zyxin, Zyxin 2

验证数据展示

  • WB analysis of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

    WB analysis of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60254-1-Ig (Zyxin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

    IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZYX antibody included
  • IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

    IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZYX antibody included
  • IF Staining of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

    IF Staining of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60254-1-Ig (Zyxin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ZYX Ready-To-Use IHC Kit. ZYX primary antibody included.

产品信息

60254-1-PBS targets Zyxin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Zyxin fusion protein Ag0419 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name zyxin
Synonyms ESP 2, HED 2, ZYX, zyxin, Zyxin 2
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC008743
Gene Symbol Zyxin
Gene ID (NCBI) 7791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15942
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.