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Alpha Internexin Polyclonal antibody

Alpha Internexin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23881-1-AP

Print datasheet

Synonyms

66 kDa neurofilament protein, Alpha internexin, Alpha Inx, INA, NEF5, Neurofilament 5, NF 66, NF-66, TXBP 1

验证数据展示

  • WB analysis using 23881-1-AP

    WB analysis using 23881-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23881-1-AP (alpha-internexin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 23881-1-AP

    IHC staining of human pancreas cancer using 23881-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 23881-1-AP (alpha-internexin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23881-1-AP targets Alpha Internexin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Alpha Internexin fusion protein Ag20844 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name internexin neuronal intermediate filament protein, alpha
Synonyms 66 kDa neurofilament protein, Alpha internexin, Alpha Inx, INA, NEF5, Neurofilament 5, NF 66, NF-66, TXBP 1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 66-68 kDa
GenBank Accession NumberBC006359
Gene Symbol INA
Gene ID (NCBI) 9118
RRIDAB_2879348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16352
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha Internexin antibody 23881-1-APDownload protocol
IHC protocol for Alpha Internexin antibody 23881-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols