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androgen receptor Monoclonal antibody, PBS Only

androgen receptor Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7C12

Cat no : 66747-1-PBS

Print datasheet

Synonyms

AR, androgen receptor,AR, NR3C4, DHTR, 1F7C12

验证数据展示

  • WB analysis using 66747-1-Ig (same clone as 66747-1-PBS)

    WB analysis using 66747-1-Ig (same clone as 66747-1-PBS)

    PC-3(AR-), DU 145(AR-) and LNCaP (AR+) cell lysates were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

    IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AR antibody included
  • IF Staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

    IF Staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using AR antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

  • IF Staining of LNCaP using 66747-1-Ig (same clone as 66747-1-PBS)

    IF Staining of LNCaP using 66747-1-Ig (same clone as 66747-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using androgen receptor antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AR Ready-To-Use IHC Kit. AR primary antibody included.

产品信息

66747-1-PBS targets androgen receptor in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen androgen receptor fusion protein Ag17291 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name androgen receptor
Synonyms AR, androgen receptor,AR, NR3C4, DHTR, 1F7C12
Calculated Molecular Weight 914 aa, 99 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC132975
Gene Symbol AR
Gene ID (NCBI) 367
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10275
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AR, also named as DHTR and NR3C4, belongs to the nuclear hormone receptor family and NR3 subfamily. AR is a ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. AR is activated, but not phosphorylated, by HIPK3. Defects in AR are the cause of androgen insensitivity syndrome (AIS), previously known as testicular feminization syndrome (TFM), which is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Defects in AR are the cause of spinal and bulbar muscular atrophy X-linked type 1 (SMAX1) which also known as Kennedy disease. Defects in AR may play a role in metastatic prostate cancer. Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) which also known as Reifenstein syndrome. AR exists various isoforms with MW 110-120 kDa and 75-80 kDa. (PMID: 19244107 )