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ChromoTek anti-IL6 recombinant VHH, biotinylated

IL6-b is a recombinant mono-biotinylated anti IL6 Nanobody (VHH), suitable for ELISA, SPR, and BLI applications.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Cat No : IL6-b

Synonyms

IL-6 VHH, IL-6 single domain antibody (sdAb), Interleukin 6, BSF-2, CDF

Validation Data Gallery

  • BLI analysis of the capture of varying concentrations of HumanKine IL-6 (HZ-1019) by biotinylated anti-IL-6 VHH (IL6-b). Please note the higher apparent affinity owing to avidity effects (dimerization of the cytokine).

    BLI analysis of the capture of varying concentrations of HumanKine IL-6 (HZ-1019) by biotinylated anti-IL-6 VHH (IL6-b). Please note the higher apparent affinity owing to avidity effects (dimerization of the cytokine).

  • ELISA analysis of the capture of varying concentrations of HumanKine IL-6 (HZ-1019) by biotinylated anti-IL-6 VHH (IL6-b). Detection via rabbit polyclonal PTG 21865-1-AP.

    ELISA analysis of the capture of varying concentrations of HumanKine IL-6 (HZ-1019) by biotinylated anti-IL-6 VHH (IL6-b). Detection via rabbit polyclonal PTG 21865-1-AP.

Product Information

IL6-b is a recombinant mono-biotinylated anti IL6 Nanobody (VHH), suitable for ELISA, SPR, and BLI applications.

ApplicationsBLI, SPR, ELISA
ReactivityHuman
ConjugateBiotin
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity3 nM
Molecular Weight13.7 kDa
FormLiquid
RRIDAB_3665394
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C
Shippingambient
BackgroundInterleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.