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beta-Catenin antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB

Cat No : 61487,61488 61487

Synonyms

beta-Catenin, catenin, Catenin (Cadherin-Associated Protein), Beta 1, 88kDa, Catenin (Cadherin-Associated Protein), Beta 1 (88kD), CTNNB1, MRD19, Armadillo, Catenin Beta-1, antibody, polyclonal, pAb, western blot, wb, sample

验证数据展示

  • beta-Catenin antibody (pAb) tested by Immunoprecipitation. 10 ul of beta-Catenin antibody was used to immunoprecipitate beta-Catenin from 500 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the beta-Catenin antibody at a dilution of 1:500.

    beta-Catenin antibody (pAb) tested by Immunoprecipitation. 10 ul of beta-Catenin antibody was used to immunoprecipitate beta-Catenin from 500 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the beta-Catenin antibody at a dilution of 1:500.

  • beta-Catenin antibody (pAb) tested by Western blot. Detection of beta-Catenin antibody by Western blot analysis. Lane 1: A549 nuclear extract (30 ug). Lane 2: DU145 nuclear extract (20 ug). Both probed with beta-Catenin antibody at a dilution of 1:500.

    beta-Catenin antibody (pAb) tested by Western blot. Detection of beta-Catenin antibody by Western blot analysis. Lane 1: A549 nuclear extract (30 ug). Lane 2: DU145 nuclear extract (20 ug). Both probed with beta-Catenin antibody at a dilution of 1:500.

产品信息

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ul per IP WB: 1:500 - 1:2,000 dilution
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of human beta-Catenin.
Full Name beta-Catenin antibody (pAb)
Synonyms beta-Catenin, catenin, Catenin (Cadherin-Associated Protein), Beta 1, 88kDa, Catenin (Cadherin-Associated Protein), Beta 1 (88kD), CTNNB1, MRD19, Armadillo, Catenin Beta-1, antibody, polyclonal, pAb, western blot, wb, sample
Molecular weight 90 kDa
GenBank accession numberNP_001895
RRIDAB_2614973
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

beta-Catenin is a key downstream component of the canonical Wnt signaling pathway, plays an important role in the regulation of cell adhesion and embryonic development. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of beta-Catenin via BTRC and subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activation of Wnt responsive genes.