Chromogranin A Monoclonal antibody

Chromogranin A Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 60135-2-Ig

产品说明书

CloneNo. 2A7D11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

CHGA, CGA, EA-92, Chromogranin-A, AL-11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inrat adrenal gland tissue, SH-SY5Y cells
Positive IHC detected inhuman pancreas cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pancreas cancer tissue
Positive IF/ICC detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy CgA/CHGA Ready-To-Use IHC Kit. CgA/CHGA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60135-2-Ig targets Chromogranin A in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat
免疫原 Chromogranin A fusion protein Ag0807 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromogranin A (parathyroid secretory protein 1)
别名 CHGA, CGA, EA-92, Chromogranin-A, AL-11
计算分子量 51 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC006459
基因名称 Chromogranin A
Gene ID (NCBI) 1113
RRIDAB_2918358
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10645
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

实验方案

Product Specific Protocols
WB protocol for Chromogranin A antibody 60135-2-IgDownload protocol
IHC protocol for Chromogranin A antibody 60135-2-IgDownload protocol
IF protocol for Chromogranin A antibody 60135-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Authors - Rong Wang
mouseIF

Stem Cell Res Ther

Expansion of mouse castration-resistant intermediate prostate stem cells in vitro

Authors - Yalan Xu
ratIF

Front Microbiol

Tongbian decoction restores intestinal microbiota and activates 5-hydroxytryptamine signaling: implication in slow transit constipation

Authors - Hongjia Li
humanIF

Front Pharmacol

Development of a small cell lung cancer organoid model to study cellular interactions and survival after chemotherapy

Authors - Chandani Sen
ratIF

Cell Tissue Res

Neurotoxic stimulation alters prosaposin levels in the salivary systems of rats

Authors - Farzana Khan
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