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citrate synthase Monoclonal antibody, PBS Only

citrate synthase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F9G6

Cat no : 67784-1-PBS

Print datasheet

Synonyms

citrate synthase, CS

验证数据展示

  • WB analysis using 67784-1-Ig (same clone as 67784-1-PBS)

    WB analysis using 67784-1-Ig (same clone as 67784-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67784-1-Ig (citrate synthase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CS antibody included
  • IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CS antibody included
  • IF Staining of HepG2 using 67784-1-Ig (same clone as 67784-1-PBS)

    IF Staining of HepG2 using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67784-1-Ig (same clone as 67784-1-PBS)

    IF Staining of MCF-7 using 67784-1-Ig (same clone as 67784-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CS Ready-To-Use IHC Kit. CS primary antibody included.

产品信息

67784-1-PBS targets citrate synthase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen citrate synthase fusion protein Ag9255 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name citrate synthase
Synonyms citrate synthase, CS
Calculated Molecular Weight 466 aa, 52 kDa
GenBank Accession NumberBC010106
Gene Symbol Citrate Synthase
Gene ID (NCBI) 1431
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75390
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.