Cleaved Caspase 3 Polyclonal antibody

Cleaved Caspase 3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25128-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (4)

应用

WB, IHC, IF/ICC, ELISA and More (1)

CASP3, Caspase3, CASP 3, CASP-3, Caspase 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Cleaved Caspase 3 Ready-To-Use IHC Kit. Cleaved Caspase 3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25128-1-AP targets Cleaved Caspase 3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, chicken, bovine, goat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase 3, apoptosis-related cysteine peptidase
别名 CASP3, Caspase3, CASP 3, CASP-3, Caspase 3
计算分子量 32 kDa
观测分子量17-25 kDa
GenBank蛋白编号NM_032991
基因名称 Caspase 3
Gene ID (NCBI) 836
RRIDAB_3073913
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42574
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize the cleaved Caspase 3 fragments. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved fragment of Caspase 3 might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

实验方案

Product Specific Protocols
WB protocol for Cleaved Caspase 3 antibody 25128-1-APDownload protocol
IHC protocol for Cleaved Caspase 3 antibody 25128-1-APDownload protocol
IF protocol for Cleaved Caspase 3 antibody 25128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Hazard Mater

Exposure to 6PPD-Q induces dysfunctions of ovarian granulosa cells: Its potential role in PCOS

Authors - Hanxi Yu
humanWB

Small

A Responsive Nanorobot Modulates Intracellular Zinc Homeostasis to Amplify Mitochondria-Targeted Phototherapy

Authors - Xinyuan Zhou
humanWB

Redox Biol

DNAJC12 causes breast cancer chemotherapy resistance by repressing doxorubicin-induced ferroptosis and apoptosis via activation of AKT

Authors - Mengjia Shen
mouseWB

J Adv Res

TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway

Authors - Kai Sun
mouseWB,IHC

Adv Healthc Mater

Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation

Authors - Ning Wang
mouseWB

Sci Total Environ

Nasal instillation of polystyrene nanoplastics induce lung injury via mitochondrial DNA release and activation of the cyclic GMP-AMP synthase-stimulator of interferon genes-signaling cascade

Authors - Jing Liu
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