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luciferase Monoclonal antibody

luciferase Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

　 and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F2C12

Cat no : 60158-1-Ig

Print datasheet

Synonyms

dNGLUC, Luciferase

验证数据展示

  • WB analysis of Recombinant protein using 60158-1-Ig

    WB analysis of Recombinant protein using 60158-1-Ig

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60158-1-Ig (dNGLUC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

60158-1-Ig targets luciferase in WB, ELISA applications and shows reactivity with 　 samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity 　
Cited Reactivityhuman, mouse
Immunogen luciferase fusion protein Ag13000 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name luciferase
Synonyms dNGLUC, Luciferase
Calculated Molecular Weight 20 kDa
GenBank Accession NumberAY015993
Gene Symbol
Gene ID (NCBI)
RRIDAB_10665950
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. The immunogen of this antibody the luciferase(AY015993) from the copepod marine organism Gaussia princeps. This antibody is a McAb to Luciferase.

实验方案

Product Specific Protocols
WB protocol for luciferase antibody 60158-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

PLoS One

Multiplex Assay for Live-Cell Monitoring of Cellular Fates of Amyloid-β Precursor Protein (APP).

Authors - Maria Merezhko
View Article
humanWB

Mol Ther

Protein expression/secretion boost by a novel unique 21-mer cis-regulatory motif (Exin21) via mRNA stabilization

Authors - Yuanjun Zhu
View Article