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domain-I-of-FIZ-1 Polyclonal antibody

domain-I-of-FIZ-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15826-1-AP

Print datasheet

Synonyms

FIZ1, ZNF798, Flt3-interacting zinc finger protein 1, FIZ 1

验证数据展示

  • WB analysis of human kidney using 15826-1-AP

    WB analysis of human kidney using 15826-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15826-1-AP (domain-I-of-FIZ-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15826-1-AP

    IF Staining of HepG2 using 15826-1-AP

    Immunofluorescent analysis of () fixed HepG2 cells using 15826-1-AP (domain-I-of-FIZ-1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human kidney using 15826-1-AP

    IHC staining of human kidney using 15826-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman kidney tissue, Y79 cells
Positive IHC detected inhuman kidney tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15826-1-AP targets domain-I-of-FIZ-1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FLT3-interacting zinc finger 1
Synonyms FIZ1, ZNF798, Flt3-interacting zinc finger protein 1, FIZ 1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberNM_032836
Gene Symbol FIZ1
Gene ID (NCBI) 84922
RRIDAB_2102547
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SL8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for domain-I-of-FIZ-1 antibody 15826-1-APDownload protocol
IHC protocol for domain-I-of-FIZ-1 antibody 15826-1-APDownload protocol
IF protocol for domain-I-of-FIZ-1 antibody 15826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols