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验证数据展示

  • WB analysis of human kidney using 15827-1-AP

    WB analysis of human kidney using 15827-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15827-1-AP (domain-II-of-FIZ1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15827-1-AP

    IHC staining of human kidney using 15827-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15827-1-AP (domain-II-of-FIZ1 Antibody) at dilution of 1:50 (under 40x lens).

domain-II-of-FIZ1 Polyclonal antibody

domain-II-of-FIZ1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15827-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

domain I of FIZ 1, domain II of FIZ1, FIZ1, FLT3 interacting zinc finger 1, Zinc finger protein 798, ZNF798

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15827-1-AP targets domain-II-of-FIZ1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FLT3-interacting zinc finger 1
别名 domain I of FIZ 1, domain II of FIZ1, FIZ1, FLT3 interacting zinc finger 1, Zinc finger protein 798, ZNF798
计算分子量 52 kDa
观测分子量 60 kDa
GenBank蛋白编号NM_032836
基因名称 FIZ1
Gene ID (NCBI) 84922
RRIDAB_2102545
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96SL8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FIZ1, also named as ZNF798, may be a transcriptional repressor of NRL function in photoreceptors. FIZ1 does not repress CRX-mediated transactivation.

实验方案

Product Specific Protocols
WB protocol for domain-II-of-FIZ1 antibody 15827-1-APDownload protocol
IHC protocol for domain-II-of-FIZ1 antibody 15827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols