• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Dynamitin (69-376 AA) Polyclonal antibody

Dynamitin (69-376 AA) Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10030-2-AP

Print datasheet

Synonyms

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, Dynamitin (69-376 AA), p50 dynamitin, RBP50

验证数据展示

  • WB analysis of mouse brain using 10030-2-AP

    WB analysis of mouse brain using 10030-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10030-2-AP (Dynamitin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 10030-2-AP

    IHC staining of human gliomas using 10030-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 10030-2-AP

    IHC staining of human gliomas using 10030-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 10030-2-AP

    IP experiment of mouse brain using 10030-2-AP

    IP result of anti-Dynamitin (69-376 AA) (IP:10030-2-AP, 4ug; Detection:10030-2-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10030-2-AP targets Dynamitin (69-376 AA) in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynactin 2 (p50)
Synonyms DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, Dynamitin (69-376 AA), p50 dynamitin, RBP50
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC000718
Gene Symbol Dynamitin
Gene ID (NCBI) 10540
RRIDAB_513348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13561
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the internal region (69-376aa) of full length p50 dynamitin.

实验方案

Product Specific Protocols
WB protocol for Dynamitin (69-376 AA) antibody 10030-2-APDownload protocol
IHC protocol for Dynamitin (69-376 AA) antibody 10030-2-APDownload protocol
IP protocol for Dynamitin (69-376 AA) antibody 10030-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols