验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse testis tissue, mouse lung tissue, human testis tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
10031-2-AP targets Dynamitin (1-83 AA) in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dynactin 2 (p50) |
| 别名 | DCTN2, DYNAMITIN, Dynamitin (1-401 AA), Dynamitin (1-86 AA), Dynamitin (200-406 aa) |
| 计算分子量 | 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC000718 |
| 基因名称 | Dynamitin |
| Gene ID (NCBI) | 10540 |
| RRID | AB_2090613 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13561 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the N terminal 59aa of full length p50 dynamitin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Dynamitin (1-83 AA) antibody 10031-2-AP | Download protocol |
| IHC protocol for Dynamitin (1-83 AA) antibody 10031-2-AP | Download protocol |
| IP protocol for Dynamitin (1-83 AA) antibody 10031-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
