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验证数据展示

  • WB analysis using 11361-1-AP

    WB analysis using 11361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11361-1-AP (Dynamitin (1-401 AA) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 11361-1-AP

    IHC staining of human stomach cancer using 11361-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11361-1-AP (Dynamitin (1-401 AA) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with DCTN2 antibody included

Dynamitin (1-401 AA) Polyclonal antibody

Dynamitin (1-401 AA) Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11361-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DCTN2, DYNAMITIN, Dynamitin (1-83 AA), Dynamitin (1-86 AA), Dynamitin (200-406 aa)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DCTN2 Ready-To-Use IHC Kit. DCTN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11361-1-AP targets Dynamitin (1-401 AA) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Dynamitin (1-401 AA) fusion protein Ag1902 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynactin 2 (p50)
别名 DCTN2, DYNAMITIN, Dynamitin (1-83 AA), Dynamitin (1-86 AA), Dynamitin (200-406 aa)
计算分子量 401 aa, 44 kDa
观测分子量 50 kDa
GenBank蛋白编号BC014083
基因名称 Dynamitin
Gene ID (NCBI) 10540
RRIDAB_2090609
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13561
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83aa is responsible for calmodulin binding. This antibody is against the full length p50 dynamitin.

实验方案

Product Specific Protocols
WB protocol for Dynamitin (1-401 AA) antibody 11361-1-APDownload protocol
IHC protocol for Dynamitin (1-401 AA) antibody 11361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols