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Dynamitin (1-401 AA) Polyclonal antibody

Dynamitin (1-401 AA) Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11361-1-AP

Print datasheet

Synonyms

DCTN2, DYNAMITIN, Dynamitin (1-83 AA), Dynamitin (1-86 AA), Dynamitin (200-406 aa)

验证数据展示

  • WB analysis using 11361-1-AP

    WB analysis using 11361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11361-1-AP (Dynamitin (1-401 AA) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 11361-1-AP

    IHC staining of human stomach cancer using 11361-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11361-1-AP (Dynamitin (1-401 AA) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

经过测试的应用

Positive WB detected inA375 cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11361-1-AP targets Dynamitin (1-401 AA) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Dynamitin (1-401 AA) fusion protein Ag1902 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynactin 2 (p50)
Synonyms DCTN2, DYNAMITIN, Dynamitin (1-83 AA), Dynamitin (1-86 AA), Dynamitin (200-406 aa)
Calculated Molecular Weight 401 aa, 44 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC014083
Gene Symbol Dynamitin
Gene ID (NCBI) 10540
RRIDAB_2090609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13561
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83aa is responsible for calmodulin binding. This antibody is against the full length p50 dynamitin.

实验方案

Product Specific Protocols
WB protocol for Dynamitin (1-401 AA) antibody 11361-1-APDownload protocol
IHC protocol for Dynamitin (1-401 AA) antibody 11361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols