验证数据展示
经过测试的应用
| Positive WB detected in | human kidney tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | zebrafish retina |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
18301-1-AP targets Collagen Type XVIII in WB, IHC, IF, ELISA applications and shows reactivity with human, zebrafish samples.
| 经测试应用 | WB, IHC, IF, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, zebrafish |
| 文献引用反应性 | human, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | collagen, type XVIII, alpha 1 |
| 别名 | COL18, COL18A1, Endostatin, Collagen alpha 1(XVIII) chain, Collagen alpha-1(XVIII) chain |
| 计算分子量 | 178 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC063833 |
| 基因名称 | COL18A1/Endostatin |
| Gene ID (NCBI) | 80781 |
| RRID | AB_2081719 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P39060 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COL18A1 is a naturally-occurring 20-kDa C-terminal fragment derived from collagen alpha-1(XVIII) chain. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. It potently inhibits endothelial cell proliferation and inhibits endothelial cell angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. COL18A1 is a broad spectrum angiogenesis inhibitor and may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF). This antibody raised against 1693-1706 aa of human collagen alpha-1(XVIII) chain can recognize endostatin (20 kDa) and several endostatin-related fragments of higher molecular weight (PMID: 15545970; 19358707).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Collagen Type XVIII antibody 18301-1-AP | Download protocol |
| IHC protocol for Collagen Type XVIII antibody 18301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. | ||
Cancer Lett Systemic administration of mesenchymal stem cells loaded with a novel oncolytic adenovirus carrying IL-24/endostatin enhances glioma therapy. | ||
FASEB J MicroRNA-3064-5p sponged by MALAT1 suppresses angiogenesis in human hepatocellular carcinoma by targeting the FOXA1/CD24/Src pathway. | ||
Neural Regen Res miR-181b promotes angiogenesis and neurological function recovery after ischemic stroke |