Endostatin Polyclonal antibody

Endostatin Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, zebrafish and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18301-1-AP

Print datasheet

Synonyms

COL18A1, Collagen alpha 1(XVIII) chain, collagen, type XVIII, alpha 1, Endostatin, KNO, KNO1



经过测试的应用

Positive WB detected inhuman kidney tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inzebrafish retina

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18301-1-AP targets Endostatin in WB, IF, IHC, ELISA applications and shows reactivity with human, zebrafish samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, zebrafish
Cited Reactivityhuman, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type XVIII, alpha 1
Synonyms COL18A1, Collagen alpha 1(XVIII) chain, collagen, type XVIII, alpha 1, Endostatin, KNO, KNO1
Calculated Molecular Weight 178 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC063833
Gene Symbol COL18A1/Endostatin
Gene ID (NCBI) 80781
RRIDAB_2081719
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP39060
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from collagen alpha-1(XVIII) chain (COL18A1). It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. It potently inhibits endothelial cell proliferation and inhibits endothelial cell angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. Endostatin is a broad spectrum angiogenesis inhibitor and may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF). This antibody raised against 1693-1706 aa of human collagen alpha-1(XVIII) chain can recognize endostatin (20 kDa) and several endostatin-related fragments of higher molecular weight (PMID: 15545970; 19358707).

实验方案

Product Specific Protocols
WB protocol for Endostatin antibody 18301-1-APDownload protocol
IHC protocol for Endostatin antibody 18301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
humanWB

Cancer Lett

Systemic administration of mesenchymal stem cells loaded with a novel oncolytic adenovirus carrying IL-24/endostatin enhances glioma therapy.

Authors - Junhe Zhang
humanWB

FASEB J

MicroRNA-3064-5p sponged by MALAT1 suppresses angiogenesis in human hepatocellular carcinoma by targeting the FOXA1/CD24/Src pathway.

Authors - Pei Zhang
humanWB

Biotechnol Lett

PMID: 26830094

Authors - Baolong Wang
ratWB

Neural Regen Res

miR-181b promotes angiogenesis and neurological function recovery after ischemic stroke

Authors - Li-Xia Xue