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fetuin-B Polyclonal antibody

fetuin-B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18052-1-AP

Print datasheet

Synonyms

16G2, FETUB, fetuin B, Fetuin like protein IRL685, fetuin-B, Gugu, IRL685

验证数据展示

  • IP experiment of human plasma using 18052-1-AP

    IP experiment of human plasma using 18052-1-AP

    IP result of anti-fetuin-B (IP:18052-1-AP, 4ug; Detection:18052-1-AP 1:2000) with human plasma lysate 4000 ug.

  • WB analysis of human plasma using 18052-1-AP

    WB analysis of human plasma using 18052-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 18052-1-AP

    IHC staining of human liver cancer using 18052-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman blood tissue, human plasma
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18052-1-AP targets fetuin-B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen fetuin-B fusion protein Ag12562 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fetuin B
Synonyms 16G2, FETUB, fetuin B, Fetuin like protein IRL685, fetuin-B, Gugu, IRL685
Calculated Molecular Weight 382 aa, 42 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC074734
Gene Symbol Fetuin B
Gene ID (NCBI) 26998
RRIDAB_2103181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGM5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fetuin-B is a protein that in humans is encoded by the FETUB gene. The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the ins and hepatocyte growth factor receptors, and response to systemic inflammation. It may be a protease inhibitor required for egg fertilization.

实验方案

Product Specific Protocols
WB protocol for fetuin-B antibody 18052-1-APDownload protocol
IHC protocol for fetuin-B antibody 18052-1-APDownload protocol
IP protocol for fetuin-B antibody 18052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols