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gp130/IL6ST Polyclonal antibody

gp130/IL6ST Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30591-1-AP

Print datasheet

Synonyms

IL6ST, IL 6R beta, IL 6 receptor subunit beta, GP130 RAPS, GP130

验证数据展示

  • WB analysis using 30591-1-AP

    WB analysis using 30591-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30591-1-AP (gp130/IL6ST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 30591-1-AP

    IF Staining of HepG2 using 30591-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using gp130/IL6ST antibody (30591-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, NCCIT cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IL6ST/GP130 Ready-To-Use IHC Kit. IL6ST/GP130 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30591-1-AP targets gp130/IL6ST in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 6 signal transducer (gp130, oncostatin M receptor)
Synonyms IL6ST, IL 6R beta, IL 6 receptor subunit beta, GP130 RAPS, GP130
Calculated Molecular Weight 104 kDa
Observed Molecular Weight130-150 kDa
GenBank Accession NumberNM_002184.3
Gene Symbol IL6ST
Gene ID (NCBI) 3572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40189-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).

实验方案

Product Specific Protocols
WB protocol for gp130/IL6ST antibody 30591-1-APDownload protocol
IF protocol for gp130/IL6ST antibody 30591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols