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验证数据展示

  • WB analysis using 84113-5-RR (same clone as 84113-5-PBS)

    WB analysis using 84113-5-RR (same clone as 84113-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84113-5-RR (gp130/IL6ST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84113-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01049-4

    Sandwich ELISA standard curve of MP01049-4

    Sandwich ELISA standard curve of MP01049-4, Rat gp130/IL6ST Recombinant Matched Antibody Pair - PBS only. 84113-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0857. 84113-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-2500 pg/mL

  • Sample test of MP01049-4

    Sample test of MP01049-4

    The mean gp130/IL6ST concentration was determined to be 24.02 ng/mL in C6 cell extract based on a 2.0 mg/mL extract load, 27.20 ng/mL in PC-12 cell extract based on a 1.4 mg/mL extract load and 16.64 ng/mL in rat brain tissue extract based on a 1.6 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84113-5-RR

    Affinity and Kinetic Characterization of 84113-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84113-5-RR against Rat gp130/IL6ST were performed. The affinity constant is 1.04 nM.

Rat gp130/IL6ST Recombinant antibody, PBS Only (Detector)

gp130/IL6ST Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84113-5-PBS

产品说明书

CloneNo. 241340A11

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, Sandwich ELISA, Indirect ELISA, Sample test

别名

IL6RB, IL-6R subunit beta, IL-6 receptor subunit beta, gp130, CD130

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84113-5-PBS targets gp130/IL6ST as part of a matched antibody pair:

MP01049-4: 84113-2-PBS capture and 84113-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 rat
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 6 signal transducer
别名 IL6RB, IL-6R subunit beta, IL-6 receptor subunit beta, gp130, CD130
计算分子量102 kDa
观测分子量90-150 kDa
GenBank蛋白编号NM_001008725.3
基因名称 Il6st
Gene ID (NCBI) 25205
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP40190
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). The binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).