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Anti-Human gp130/IL6ST Rabbit Recombinant Antibody

gp130/IL6ST Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240992H1

Cat no : 98121-1-RR

Print datasheet

Synonyms

IL6ST, IL 6 receptor subunit beta, GP130 RAPS, GP130, Glycoprotein 130

验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98121-1-RR

    FC experiment of human peripheral blood leukocytes using 98121-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human gp130/IL6ST Rabbit Recombinant Antibody (98121-1-RR, Clone: 240992H1) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98121-1-RR

    FC experiment of human peripheral blood leukocytes using 98121-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human gp130/IL6ST Rabbit Recombinant Antibody (98121-1-RR, Clone: 240992H1) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98121-1-RR

    Affinity and Kinetic Characterization of 98121-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98121-1-RR against Human gp130/IL6ST were performed. The affinity constant is 115 nM.

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy IL6ST/GP130 Ready-To-Use IHC Kit. IL6ST/GP130 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98121-1-RR is gp130/IL6ST Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 signal transducer (gp130, oncostatin M receptor)
Synonyms IL6ST, IL 6 receptor subunit beta, GP130 RAPS, GP130, Glycoprotein 130
Calculated Molecular Weight 104 kDa
GenBank Accession NumberNM_002184.3
Gene Symbol IL6ST
Gene ID (NCBI) 3572
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP40189-1
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3.

实验方案

Product Specific Protocols
FC protocol for gp130/IL6ST antibody 98121-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols