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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98121-1-RR

    FC experiment of human peripheral blood leukocytes using 98121-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human gp130/IL6ST Rabbit Recombinant Antibody (98121-1-RR, Clone: 240992H1) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98121-1-RR

    FC experiment of human peripheral blood leukocytes using 98121-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human gp130/IL6ST Rabbit Recombinant Antibody (98121-1-RR, Clone: 240992H1) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98121-1-RR

    Affinity and Kinetic Characterization of 98121-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98121-1-RR against Human gp130/IL6ST were performed. The affinity constant is 115 nM.

Anti-Human gp130/IL6ST Rabbit Recombinant Antibody

gp130/IL6ST Uni-rAbTM Recombinant Antibody for FC
Cat No. 98121-1-RR

产品说明书

CloneNo. 240992H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

IL6ST, 240992H1, CD130, Glycoprotein 130, GP130

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy IL6ST/GP130 Ready-To-Use IHC Kit. IL6ST/GP130 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98121-1-RR targets gp130/IL6ST in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 6 signal transducer (gp130, oncostatin M receptor)
别名 IL6ST, 240992H1, CD130, Glycoprotein 130, GP130
计算分子量 104 kDa
GenBank蛋白编号NM_002184.3
基因名称 IL6ST
Gene ID (NCBI) 3572
RRIDAB_3672268
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP40189-1
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3.

实验方案

Product Specific Protocols
FC protocol for gp130/IL6ST antibody 98121-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols