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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

    WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IST1 antibody included
  • IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IST1 antibody included
  • IF Staining of HeLa using 66989-1-Ig (same clone as 66989-1-PBS)

    IF Staining of HeLa using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using hIST1 antibody (66989-1-Ig, Clone: 1E12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

hIST1 Monoclonal antibody, PBS Only

hIST1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66989-1-PBS

产品说明书

CloneNo. 1E12A7

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IST1 Ready-To-Use IHC Kit. IST1 primary antibody included.

产品信息

66989-1-PBS targets hIST1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 hIST1 fusion protein Ag28364 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA0174
别名 hIST1, IST1, IST1 homolog, KIAA0174, OLC1
计算分子量 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC000430
基因名称 hIST1
Gene ID (NCBI) 9798
RRIDAB_2882306
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP53990
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.