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hIST1 Monoclonal antibody, PBS Only

hIST1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1E12A7

Cat No : 66989-1-PBS

Print datasheet

Synonyms

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

验证数据展示

  • WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

    WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66989-1-Ig (same clone as 66989-1-PBS)

    IF Staining of HeLa using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using hIST1 antibody (66989-1-Ig, Clone: 1E12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

产品信息

66989-1-PBS targets hIST1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen hIST1 fusion protein Ag28364 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name KIAA0174
Synonyms hIST1, IST1, IST1 homolog, KIAA0174, OLC1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC000430
Gene Symbol KIAA0174
Gene ID (NCBI) 9798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53990
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.