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hnRNP-E1 Polyclonal antibody
hnRNP-E1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, MCF-7 cells, HeLa cells, HepG2 cells, NIH/3T3 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, NIH/3T3 cells, A431 cells, sodium arsenite treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PCBP1 Ready-To-Use IHC Kit. PCBP1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14523-1-AP targets hnRNP-E1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | hnRNP-E1 fusion protein Ag6007 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | poly(rC) binding protein 1 |
| Synonyms | hnRNP E1/PCBP1, hnRNP E1, PCBP1, Nucleic acid-binding protein SUB2.3, HNRPE1 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC039742 |
| Gene Symbol | PCBP1 |
| Gene ID (NCBI) | 5093 |
| RRID | AB_2160619 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15365 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
hnRNP-E1 (heterogeneous nuclear ribonucleoprotein E1) is also named as PCBP1 and Alpha-CP1. Poly(rC)-binding protein 1 (PCBP1 also called hnRNP E1) belongs to a hnRNP family that is composed of hnRNP K/J and the alpha-complex proteins (PCBP1-4α or CP1-4). Proteins in this family contain three hnRNP K homology (KH) domains for RNA-binding. The human PCBP1 gene encodes the hnRNP E1 protein, and was initially defined as clone sub2.3, with poly(C)-binding activity and observed similarity to hnRNP E2 (PMID: 8152927). hnRNP-E1 is an RNA binding protein that regulates diverse processes including translation and splicing (PMID:29396052). hnRNP E1 regulates splicing of EMT-associated genes including CD44 and PNUTS (PMID: 28825698). This antibody recognizes hnRNP E1/E2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for hnRNP-E1 antibody 14523-1-AP | Download protocol |
| IHC protocol for hnRNP-E1 antibody 14523-1-AP | Download protocol |
| IF protocol for hnRNP-E1 antibody 14523-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation. | ||
Nucleic Acids Res G-Quadruplex binding enantiomers show chiral selective interactions with human telomere. | ||
Bioengineered PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma. | ||
Mol Cell Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X. | ||
