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Recombinant Human 4-1BB Ligand/TNFSF9 protein (hFc Tag)

种属

Human

纯度

>95 %, SDS-PAGE

标签

hFc Tag

生物活性

EC50: 6-24 ng/mL

Cat no : Eg0079

Print datasheet

Synonyms

4-1BB Ligand/TNFSF9, TNFSF9, 4 1BB L, 4 1BB ligand, 4 1BBL

验证数据展示

  • Purity of Recombinant Human 4-1BB Ligand was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human 4-1BB Ligand was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human 4-1BB (Myc tag, His tag) at 0.5 μg/mL (100 μL/well) can bind Human 4-1BB Ligand (hFc tag) with a linear range of 6-24 ng/mL.

    Immobilized Human 4-1BB (Myc tag, His tag) at 0.5 μg/mL (100 μL/well) can bind Human 4-1BB Ligand (hFc tag) with a linear range of 6-24 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Human 4-1BB (Myc tag, His tag) at 0.5 μg/mL (100 μL/well) can bind Human 4-1BB Ligand (hFc tag) with a linear range of 6-24 ng/mL.
来源 HEK293-derived Human 4-1BB Ligand protein Arg71-Glu254 (Accession# P41273) with a human IgG1 Fc tag at the N-terminus.
基因ID 8744
蛋白编号 P41273
预测分子量 45.4 kDa
SDS-PAGE 45-50 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

TNFSF9 (Tumor necrosis factor ligand superfamily member 9) is also known as 4-1BBL (4-1BB ligand) or CD137L. TNFSF9 is a type 2 transmembrane glycoprotein receptor that is found on APCs (antigen presenting cells). TNFSF9 is expressed on activated T Lymphocytes. The TNFSF9/4-1BB complex with the help of T-cell receptor signals can trigger the increase in CD28− T cells and inhibit tumor growth. The interaction between 4-1BB and TNFSF9 provides costimulatory signals to T cells, which can be used to cancer immunotherapy.

参考文献:

1.Jacob Bukczynski. et al. (2003). Eur J Immunol. 33(2): 446-54.
2.Adam T C Cheuk. et al. (2004). Cancer Gene Ther. 11(3): 215-26.
3.Chao Wang. et al. (2009). Immunol Rev. 229(1): 192-215.
4.Dass S Vinay. et al. (2012). Mol Cancer Ther. 11(5): 1062-1070.
5.Cariad Chester. et al. (2018). Blood. 131(1): 49-57.