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Recombinant Human CD16(F176V) protein (Myc Tag, His Tag)

ED50

0.5-2 μg/mL

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

2214

Accession

P08637

Cat No : Eg31662

Print datasheet

Synonyms

CD16, CD16A, Fc gamma RIII alpha, FCG3, FCGR3, FCGR3A, FCGRIII, FCRIII, FCRIIIA, IGFR3

验证数据展示

  • Purity of Recombinant Human CD16 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD16 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human CD16 (F176V) (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human IgG1 with a linear range of 0.5-2 μg/mL.

    Immobilized Human CD16 (F176V) (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human IgG1 with a linear range of 0.5-2 μg/mL.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human CD16 (F176V) (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human IgG1 with a linear range of 0.5-2 μg/mL.
Source HEK293-derived Human CD16 protein Gly17-Gln208 (Accession# P08637, F176V) with a Myc tag and a His tag at the C-terminus.
Predicted Molecular Mass 24.3 kDa
SDS-PAGE 38-60 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD16, also known as the low affinity Fc gamma receptor III for IgG (FcγRIII), exists in two isoforms, FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. CD16a is expressed on NK cells, macrophages, and placental trophoblasts as a polypeptide-anchored transmembrane protein while CD16b is expressed on neutrophils in a glycosylphosphatidylinositol (GPI)-anchored form. CD16a forms a heteromeric structure with the Fc epsilon RI (gamma) and/or CD3 (zeta) subunits. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

References:

1. S Nagarajan, et al. (1995) J Biol Chem. 270(43):25762-70.
2. J E Gessner, et al. (1995) J Biol Chem. 270(3):1350-61.