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Recombinant Human CD2 protein (Myc Tag, His Tag)

ED50

30-120 ng/mL

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

914

Accession

P06729

Cat No : Eg0138

Print datasheet

Synonyms

CD2, LFA-2, SRBC, T11

验证数据展示

  • Purity of Recombinant Human CD2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human CD58 (His tag) at 0.5 μg/mL (100 μL/well) can bind Human CD2 (Myc tag, His tag) with a linear range of 30-120 ng/mL.

    Immobilized Human CD58 (His tag) at 0.5 μg/mL (100 μL/well) can bind Human CD2 (Myc tag, His tag) with a linear range of 30-120 ng/mL.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human CD58 (His tag) at 0.5 μg/mL (100 μL/well) can bind Human CD2 (Myc tag, His tag) with a linear range of 30-120 ng/mL.
Source HEK293-derived Human CD2 protein Lys25-Asp209 (Accession# P06729) with a Myc tag and a His tag at the C-terminus.
Predicted Molecular Mass 23.8 kDa
SDS-PAGE 40-48 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD2 (also known as LFA-2, T-cell surface antigen T11/Leu-5, LFA-3 receptor, E-rosette receptor) is a member of the immunoglobulin superfamily. It is a transmembrane glycoprotein present on T cells, NK cells, thymocytes and dendritic cells. CD2 functions as an adhesion molecule, binding with lymphocyte function-associated antigen (LFA-3/CD58) in humans. It has also been reported to interact with CD48 and CD59. CD2 plays important roles in cell adhesion between T-cells/NK cells and other cell types, T-cell activation, NK-cell activation, and thymocyte development.

References:

1. P A van der Merwe, et al. (1994) Biochemistry. 33(33):10149-60.
2. S J Davis, et al. (1996) Immunol Today. 17(4):177-87.
3. A L Wilkins, et al. (2003) Curr Protein Pept Sci. 4(5):367-73. 
4. Christian Binder, et al. (2020) Front Immunol. 11:1090.