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Recombinant Human CD27 protein (hFc Tag, Myc Tag, His Tag)

ED50

20-80 ng/mL

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

939

Accession

P26842

Cat No : Eg0037

Print datasheet

Synonyms

CD27, S152, T14, TNFRSF7, Tp55

验证数据展示

  • Purity of Recombinant Human CD27 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD27 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human CD70 (His tag) at 2 μg/mL (100 μL/well) can bind Human CD27 (hFc tag, Myc tag, His tag) with a linear range of 20-80 ng/mL.

    Immobilized Human CD70 (His tag) at 2 μg/mL (100 μL/well) can bind Human CD27 (hFc tag, Myc tag, His tag) with a linear range of 20-80 ng/mL.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human CD70 (His tag) at 2 μg/mL (100 μL/well) can bind Human CD27 (hFc tag, Myc tag, His tag) with a linear range of 20-80 ng/mL.
Source HEK293-derived Human CD27 protein Ala20-Arg191 (Accession# P26842) with a human IgG1 Fc tag, a Myc tag and a His tag at the C-terminus.
Predicted Molecular Mass 47.7 kDa
SDS-PAGE 58-70 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity. It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway. CD27 may also play a role in apoptosis through association with SIVA1.

References:

1. K V Prasad, et al. (1997) Proc Natl Acad Sci U S A. 94(12):6346-51.
2. J Hendriks, et al. (2000) Nat Immunol. 1(5):433-40. 
3. Martijn A Nolte, et al. (2009) Immunol Rev. 229(1):216-31.