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Recombinant Human CEACAM-1/CD66a protein (His Tag)

ED50

70-280 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

634

Accession

P13688-1

Cat No : Eg0314

Print datasheet

Synonyms

CEACAM1,CEACAM1/CD66a,BGP,BGP1,BGP-1

验证数据展示

  • Purity of Recombinant Human CEACAM-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CEACAM-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human CEACAM-1 (His tag) at 2 μg/mL (100 μL/well) can bind Human CEACAM-8 (hFc tag) with a linear range of 70-280 ng/mL.

    Immobilized Human CEACAM-1 (His tag) at 2 μg/mL (100 μL/well) can bind Human CEACAM-8 (hFc tag) with a linear range of 70-280 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human CEACAM-1 (His tag) at 2 μg/mL (100 μL/well) can bind Human CEACAM-8 (hFc tag) with a linear range of 70-280 ng/mL.
Source HEK293-derived Human CEACAM-1 protein Gln35-Gly428 (Accession# P13688-1) with a His tag at the C-terminus.
Predicted Molecular Mass 47.5 kDa
SDS-PAGE 55-90 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), belonging to the immunoglobulin superfamily. It is cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner. CEACAM1is expressed incolumnar epithelial cells of the colon, T cells, granulocytes and lymphocytes. It plays a role as coinhibitory receptor in immune response, insulin action and functions also as an activator during angiogenesis. Its coinhibitory receptor function is phosphorylation- and PTPN6 -dependent, which in turn, suppress signal transduction of associated receptors by dephosphorylation of their downstream effectors. Plays a role in immune response, of T cells, natural killer (NK) and neutrophils.It inhibits cell migration and cell scattering through interaction with FLNA and interferes with the interaction of FLNA with RALA.

References:

1.Huang YH, et al. (2015). Nature. 517(7534):386–390
2.Hosomi S, et al. (2013). Eur J Immunol. 43(9):2473-2483
3.Chen Z, et al. (2008). J Immunol.180(9):6085-6093
4.Klaile E, et al. (2005). J Cell Sci. 118(Pt 23):5513–5524