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Recombinant Human CEACAM6 protein (Myc Tag, His Tag)

ED50

12-46 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

4680

Accession

P40199

Cat No : Eg0056

Print datasheet

Synonyms

CD66c, CEACAM6, CEAL, NCA, Normal cross reacting antigen

验证数据展示

  • Purity of Recombinant Human CEACAM6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CEACAM6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human CEACAM6 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CEACAM8 (hFc tag) with a linear range of 12-46 ng/mL.

    Immobilized Human CEACAM6 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CEACAM8 (hFc tag) with a linear range of 12-46 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human CEACAM6 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CEACAM8 (hFc tag) with a linear range of 12-46 ng/mL.
Source HEK293-derived Human CEACAM6 protein Lys35 -Gly320 (Accession# P40199) with a Myc tag and a His tag at the C-terminus.
Predicted Molecular Mass 33.8 kDa
SDS-PAGE 42-65 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), belonging to the immunoglobulin superfamily, is cell-adhesion protein on neutrophils. CEACAM6 is expressed in neutrophils and numerous tumor cell lines. CEACAM6 mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8. It plays a role in neutrophil adhesion to cytokine-activated endothelial cells and plays a role as an oncogene by promoting tumor progression; positively regulates cell migration, cell adhesion to endothelial cells and cell invasion. CEACAM6 is also involved in the metastatic cascade process by inducing gain resistance to anoikis of pancreatic adenocarcinoma and colorectal carcinoma cells.

References:

1.Kuroki M, et al. (2001). Journal of leukocyte biology. 70(4): 543-50 
2.Kuijpers TW, et al. (1992). J Cell Biol. 118(2):457-66
3.Blumenthal RD, et al. (2005). Cancer research. 65(19):8809–8817
4.Ordoñez C, et al. (2000). Cancer Res. 60(13):3419-24
5.Duxbury MS, et al. (2004). Oncogene. 23(2):465-73