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Recombinant Human CEACAM-8 protein (Myc Tag, His Tag)

种属

Human

纯度

>95 %, SDS-PAGE

标签

Myc Tag, His Tag

生物活性

EC50: 75-300 ng/mL

Cat no : Eg32133

Print datasheet

Synonyms

CD66b, CEACAM8, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b

验证数据展示

  • Purity of Recombinant Human CEACAM-8 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CEACAM-8 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human CEACAM-8 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-6 (Myc tag, His tag) with a linear range of 75-300 ng/mL.

    Immobilized Human CEACAM-8 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-6 (Myc tag, His tag) with a linear range of 75-300 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Human CEACAM-8 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-6 (Myc tag, His tag) with a linear range of 75-300 ng/mL.
来源 HEK293-derived Human CEACAM-8 protein Gln35-Ser319 (Accession# P31997) with a Myc tag and a His tag at the C-terminus.
基因ID 1088
蛋白编号 P31997
预测分子量 36.4 kDa
SDS-PAGE 55-70 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

A glycosylphosphatidyl-inositol-(GPI)-anchored glycoprotein, carcinoembryonic antigen (CEA)-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, NCA-95 and CD67, was identified in human neutrophils. It is solely expressed by granulocytes, of which 95% are polymorphonuclear neutrophils (PMN). CEACAM8 is cell-adhesion proteins on neutrophils that belong to the human carcinoembryonic antigen (CEA) family. CEA family members are believed to function as intercellular adhesion molecules in vitro. CEACAM8 has been shown to adhere to the closely related molecule CEACAM6 (NCA-50/90 and CD66c), suggesting a role in the interaction between granulocytes or between granulocytes and epithelial cells expressing CEACAM6 in vivo.

参考文献:

1.F Buchegger. et al. (1984). Int J Cancer. 33(5):643-649.
2.Linshu Zhao. et al. (2004). Br J Haematol. 125(5):666-673.
3.M Kuroki. et al. (2001). J Leukoc Biol. 70(4):543-550.
4.S Oikawa. et al. (1991). J Biol Chem. 266(13):7995-8001.
5.T Yamanka. et al. (1996). Biochem Biophys Res Commun.219(3):842-847.