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Recombinant Human CEACAM8 protein (Myc Tag, His Tag)

ED50

75-300 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

1088

Accession

P31997

Cat No : Eg32133

Print datasheet

Synonyms

CD66b, CD67, CEACAM8, CGM6, NCA-95

验证数据展示

  • Purity of Recombinant Human CEACAM8 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CEACAM8 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human CEACAM8 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM6 (Myc tag, His tag) with a linear range of 75-300 ng/mL.

    Immobilized Human CEACAM8 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM6 (Myc tag, His tag) with a linear range of 75-300 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human CEACAM8 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM6 (Myc tag, His tag) with a linear range of 75-300 ng/mL.
Source HEK293-derived Human CEACAM8 protein Gln35-Ser319 (Accession# P31997) with a Myc tag and a His tag at the C-terminus.
Predicted Molecular Mass 33.9 kDa
SDS-PAGE 55-70 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

A glycosylphosphatidyl-inositol-(GPI)-anchored glycoprotein, carcinoembryonic antigen (CEA)-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, NCA-95 and CD67, was identified in human neutrophils. It is solely expressed by granulocytes, of which 95% are polymorphonuclear neutrophils (PMN). CEACAM8 is cell-adhesion proteins on neutrophils that belong to the human carcinoembryonic antigen (CEA) family. CEA family members are believed to function as intercellular adhesion molecules in vitro. CEACAM8 has been shown to adhere to the closely related molecule CEACAM6 (NCA-50/90 and CD66c), suggesting a role in the interaction between granulocytes or between granulocytes and epithelial cells expressing CEACAM6 in vivo.

References:

1.F Buchegger. et al. (1984). Int J Cancer. 33(5):643-649.
2.Linshu Zhao. et al. (2004). Br J Haematol. 125(5):666-673.
3.M Kuroki. et al. (2001). J Leukoc Biol. 70(4):543-550.
4.S Oikawa. et al. (1991). J Biol Chem. 266(13):7995-8001.
5.T Yamanka. et al. (1996). Biochem Biophys Res Commun.219(3):842-847.