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Recombinant Human Galectin-3 protein (His Tag)

ED50

3-12 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

3958

Accession

P17931

Cat No : Eg0227

Print datasheet

Synonyms

35 kDa lectin, CBP35, GAL3, GALBP, Galectin-3, GALIG, L-31, Lectin L-29, LGALS2, LGALS3, MAC2

验证数据展示

  • Purity of Recombinant Human Galectin-3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Galectin-3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human Galectin-3 (His tag) at 0.5 μg/mL (100 μL/well) can bind Human CD146 (GST tag) with a linear range of 3-12 ng/mL.

    Immobilized Human Galectin-3 (His tag) at 0.5 μg/mL (100 μL/well) can bind Human CD146 (GST tag) with a linear range of 3-12 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human Galectin-3 (His tag) at 0.5 μg/mL (100 μL/well) can bind Human CD146 (GST tag) with a linear range of 3-12 ng/mL.
Source HEK293-derived Human Galectin-3 protein Ala2-Ile250 (Accession# P17931) with a His tag at the C-terminus.
Predicted Molecular Mass 26.8 kDa
SDS-PAGE 32-45 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides. Galectin-3, a 31-kDa member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers. Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space. Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis. Elevated serum galectin-3 levels have been reported in patients with breast, gastrointestinal, lung, or ovarian cancer, melanoma, and non-Hodgkin's lymphoma.

References:

1. Barondes SH, et al. (1994) J Biol Chem. 269(33):20807-10.
2. Iurisci I, et al. (2000)  Clin Cancer Res. 6(4):1389-93.
3. Takenaka Y, et al. (2002) Glycoconj J. 19(7-9):543-9.
4. Dumic J, et al.(2006) Biochim Biophys Acta. 1760(4):616-35.