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human IgD Monoclonal antibody, PBS Only

human IgD Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D1B12

Cat no : 67538-1-PBS

Print datasheet

Synonyms

FLJ00382, FLJ46727, IGHD

验证数据展示

  • WB analysis of human plasma using 67538-1-Ig (same clone as 67538-1-PBS)

    WB analysis of human plasma using 67538-1-Ig (same clone as 67538-1-PBS)

    human plasma (0.1 ul) were subjected to SDS PAGE followed by western blot with 67538-1-Ig (human IgD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgD antibody included
  • IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgD antibody included
  • IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgD antibody included
  • IF Staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IF Staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IGHD antibody (67538-1-Ig, Clone: 1D1B12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy human IgD Ready-To-Use IHC Kit. human IgD primary antibody included.

产品信息

67538-1-PBS targets human IgD in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen human IgD fusion protein Ag9614 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant delta
Synonyms FLJ00382, FLJ46727, IGHD
Calculated Molecular Weight 573 aa, 63 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC021276
Gene Symbol IGHD
Gene ID (NCBI) 3495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .