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human IgD Monoclonal antibody, PBS Only

human IgD Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1D1B12

Cat No : 67538-1-PBS

Print datasheet

Synonyms

FLJ00382, FLJ46727, IGHD

验证数据展示

  • WB analysis of human plasma using 67538-1-Ig (same clone as 67538-1-PBS)

    WB analysis of human plasma using 67538-1-Ig (same clone as 67538-1-PBS)

    human plasma (0.1 ul) were subjected to SDS PAGE followed by western blot with 67538-1-Ig (human IgD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgD antibody included
  • IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgD antibody included
  • IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgD antibody included
  • IF Staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IF Staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IGHD antibody (67538-1-Ig, Clone: 1D1B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy human IgD Ready-To-Use IHC Kit. human IgD primary antibody included.

产品信息

67538-1-PBS targets human IgD in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen human IgD fusion protein Ag9614 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant delta
Synonyms FLJ00382, FLJ46727, IGHD
Calculated Molecular Weight 573 aa, 63 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC021276
Gene Symbol IGHD
Gene ID (NCBI) 3495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .