Recombinant Human NCAM-1/CD56 protein (Myc Tag, His Tag)
ED50
/
Species
human
Purity
>90 %, SDS-PAGE
GeneID
4684
Accession
P13591-2
验证数据展示
Technical Specifications
| Purity | >90 %, SDS-PAGE |
| Endotoxin Level | <1.0 EU/μg protein, LAL method |
| Biological Activity |
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| Source | HEK293-derived Human NCAM-1 protein Leu20-Gly718 (Accession# P13591-2) with a Myc tag and a His tag at the C-terminus. |
| Predicted Molecular Mass | 79.9 kDa |
| SDS-PAGE | 80-110 kDa, reducing (R) conditions |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas.
References:
1. Shukrun R. et al. (2019). Oncogenesis. 8(9):48. 2. Sasca D. et al. (2019). Blood. 133(21):2305-2319. 3. Gunesch JT. et al. (2020). Elife. 9:e57346. 4. Sekiguchi Y. et al. (2013). J Clin Exp Hematop. 53(1):37-47.