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Recombinant Human OX40/TNFRSF4 protein (Myc Tag, His Tag)

ED50

0.3-1.2 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

7293

Accession

P43489

Cat No : Eg0022

Print datasheet

Synonyms

ACT35, CD134, OX40, OX40L receptor, TNFRSF4, TXGP1L

验证数据展示

  • Purity of Recombinant Human OX40 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human OX40 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human OX40 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human OX40L (hFc tag) with a linear range of 0.3-1.2 ng/mL.

    Immobilized Human OX40 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human OX40L (hFc tag) with a linear range of 0.3-1.2 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human OX40 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human OX40L (hFc tag) with a linear range of 0.3-1.2 ng/mL.
Source HEK293-derived Human OX40 protein Leu29-Ala214 (Accession# P43489) with a Myc tag and a His tag at the C-terminus.
Predicted Molecular Mass 22.5 kDa
SDS-PAGE 38-50 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD134 (OX40, TNFRSF4) is a member of the TNFR-superfamily of receptors. Recent evidence indicates that OX40-OX40L coupling is critical for cell survival and proliferation. While OX40 is expressed predominantly on T-lymphocytes early after antigen activation, OX40L is expressed on activated antigen presenting cells and endothelial cells within acute inflammatory environments. The tumour necrosis factor receptor OX40 (CD134) is activated by its cognate ligand OX40L (CD134L, CD252) and functions as a T cell co-stimulatory molecule. OX40 is expressed on activated T cells, and in the mouse at rest on regulatory T cells (Treg). CD134 can interact with TRAF2, TRAF3, and TRAF5. OX40 is an immune checkpoint in cancer and its presence in cancer is a good prognosis, making it a highly relevant target for the development of new immunotherapies.

References:

1.Wu LY. et al. (2020). Exp Neurol. 326:113179.
2.Gough MJ. et al. (2009). Adv Exp Med Biol. 647:94-107.
3.Webb GJ. et al. (2016). Clin Rev Allergy Immunol. 50(3):312-32. 
4.Cebada J. et al. (2021). Expert Opin Ther Pat. 31(1):81-90.