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Recombinant Human Transferrin R/CD71 protein (His Tag)

ED50

54-216 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

7037

Accession

CAA25527

Cat No : Eg0339

Print datasheet

Synonyms

CD71, p90, T9, TFR, TFR1, TFRC, TR, Transferrin R, TRFR

验证数据展示

  • Purity of Recombinant Human Transferrin R was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Transferrin R was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human Transferrin R (His tag) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin (Myc tag, His tag) with a linear range of 54-216 ng/mL.

    Immobilized Human Transferrin R (His tag) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin (Myc tag, His tag) with a linear range of 54-216 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human Transferrin R (His tag) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin (Myc tag, His tag) with a linear range of 54-216 ng/mL.
Source HEK293-derived Human Transferrin R protein Cys89-Phe760 (Accession# CAA25527) with a His tag at the N-terminus.
Predicted Molecular Mass 76 kDa
SDS-PAGE 75-90 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD71 also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is almost ubiquitously expressed, with the highest expression levels on some cells and tissues, including immature erythroid cells, placental tissue, and rapidly dividing cells. CD71 is involved in iron (Fe3+) uptake, and expression is regulated by the metabolic demand for iron. CD71 is present in actively proliferating cells and is essential for iron transport into proliferating cells.

References:

1. Speeckaert MM. et al. (2010). Crit Rev Clin Lab Sci. 47(5-6):213-228.
2. Dong HY. et al. (2011). Am J Surg Pathol. 35(5):723-732.
3. Jabara HH. et al. (2016). Nat Genet. 48(1):74-78.
4. Senyilmaz D. et al. (2015). Nature. 525(7567):124-128.