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Recombinant Human Transferrin R/CD71 protein (His Tag)

种属

Human

纯度

>95 %, SDS-PAGE

标签

His Tag

生物活性

EC50: 54-216 ng/mL

Cat no : Eg0339

Print datasheet

Synonyms

Transferrin R/CD71, CD71, p90, sTfR, T9

验证数据展示

  • Purity of Recombinant Human Transferrin R was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Transferrin R was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human Transferrin R (His tag) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin (Myc tag, His tag) with a linear range of 54-216 ng/mL.

    Immobilized Human Transferrin R (His tag) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin (Myc tag, His tag) with a linear range of 54-216 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Human Transferrin R (His tag) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin (Myc tag, His tag) with a linear range of 54-216 ng/mL.
来源 HEK293-derived Human Transferrin R protein Cys89-Phe760 (Accession# CAA25527) with a His tag at the N-terminus.
基因ID 7037
蛋白编号 CAA25527
预测分子量 76 kDa
SDS-PAGE 75-90 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

CD71 also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is almost ubiquitously expressed, with the highest expression levels on some cells and tissues, including immature erythroid cells, placental tissue, and rapidly dividing cells. CD71 is involved in iron (Fe3+) uptake, and expression is regulated by the metabolic demand for iron. CD71 is present in actively proliferating cells and is essential for iron transport into proliferating cells.

参考文献:

1. Speeckaert MM. et al. (2010). Crit Rev Clin Lab Sci. 47(5-6):213-228.
2. Dong HY. et al. (2011). Am J Surg Pathol. 35(5):723-732.
3. Jabara HH. et al. (2016). Nat Genet. 48(1):74-78.
4. Senyilmaz D. et al. (2015). Nature. 525(7567):124-128.