• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Human VEGF121 protein (Myc tag, His tag)

ED50

300-1230 ng/mL

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

7422

Accession

P15692

Cat No : Eg0192

Print datasheet

Synonyms

MVCD1, VEGF121, VEGFA, VPF

验证数据展示

  • Purity of Recombinant Human VEGF121 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human VEGF121 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human VEGF121 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human VEGFR1 (His tag) with a linear range of 300-1230 ng/mL.

    Immobilized Human VEGF121 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human VEGFR1 (His tag) with a linear range of 300-1230 ng/mL.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human VEGF121 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human VEGFR1 (His tag) with a linear range of 300-1230 ng/mL.
Source HEK293-derived Human VEGF121 protein Ala27-Arg147 (Accession# P15692) with a Myc tag and a His tag at the C-terminus.
Predicted Molecular Mass 16.9 kDa
SDS-PAGE 20-24 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression. VEGF exists in several isoforms with different molecular weights and biological properties containing 121, 145, 165, 189, and 206 amino acids.

References:

1. Senger DR. et al. (1983). Science. 219: 983-5.
2. Ferrara N. et al. (1992). Endocr Rev. 13: 18-32.
3. Boocock CA. et al. (1995). J Natl Cancer Inst. 87: 506-516.
4. Sunderkotter C. et al. (1994). Int J Cancer. 55: 410-422.
5. T Cohen. et al. (1995) J Biol Chem. 270(19):11322-6.