m6A Monoclonal antibody

m6A Monoclonal Antibody for RIP, IHC, ELISA, Dot Blot

Host / Isotype

Mouse / IgG3

Reactivity

m6A, Human, Mouse, Rat and More (5)

Applications

RIP, IHC, ELISA, Dot Blot and More (3)

Conjugate

Unconjugated

CloneNo.

1D5E10

Cat no : 68055-1-Ig

Print datasheet

Synonyms

6-N-Methyladenoside, m6A, N6-methyladenosine



经过测试的应用

Positive RIP detected inRNA
Positive IHC detected inmouse testis tissue, human lung cancer tissue, human breast cancer tissue, human colon cancer tissue, rat lymph node
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive ELISA detected inm6A
Positive Dot Blot detected inRNA

推荐稀释比

ApplicationDilution
RImmunoprecipitation (IP)RIP : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:2000-1:20000
DOT BLOTDOT BLOT : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68055-1-Ig targets m6A in WB, IHC, IF, IP, RIP, Dot Blot, ELISA applications and shows reactivity with m6A, Human, Mouse, Rat samples.

Tested Applications RIP, IHC, ELISA, Dot Blot Application Description
Cited ApplicationsWB, IHC, IF, IP, RIP, ELISA
Tested Reactivity m6A, Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat, pig, monkey
Immunogen Compound 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name m6A
Synonyms 6-N-Methyladenoside, m6A, N6-methyladenosine
GenBank Accession Numberm6A
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918796
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

m6A (N6-methyladenosine) is the most abundant internal modification in mammalian mRNA. This modification is installed by the m6A methyltransferases or termed "writers"such as METTL3 and METTL14, and can be reversed by demethylases that serve as "erasers" such as FTO and ALKBH5. The stability of m6A-modified mRNA is regulated by m6A reader protein YTHDFs, which recognizes m6A and reduces the stability of target transcripts. m6A modification and its regulatory proteins play critical roles in cancer pathogenesis and progression. m6A modification is also invovled in viruses life cycles, suggesting that drugs targets to m6A pathway could be used for antiviral thereapy.

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68055-1-IgDotBlot.pdf

实验方案

Product Specific Protocols
IHC protocol for m6A antibody 68055-1-IgDownload protocol
IP protocol for m6A antibody 68055-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Cell Biol

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Authors - Yulin Chen
humanRIP

Adv Sci (Weinh)

RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation

Authors - Yu Sun
mouseRIP,ELISA

Adv Sci (Weinh)

PXR Activation Relieves Deoxynivalenol-Induced Liver Oxidative Stress Via Malat1 LncRNA m6A Demethylation

Authors - Yue Feng
RIP

Oncogene

N6-methyladenosine reader YTHDF2 promotes multiple myeloma cell proliferation through EGR1/p21cip1/waf1/CDK2-Cyclin E1 axis-mediated cell cycle transition

Authors - Rui Liu
humanRIP

Am J Cancer Res

IGF2BP3-mediated regulation of GLS and GLUD1 gene expression promotes treg-induced immune escape in human cervical cancer

Authors - Tiantian Zhou

J Gerontol A Biol Sci Med Sci

METTL3 promotes nucleus pulposus cell senescence in intervertebral disc degeneration by regulating TLR2 m6A methylation and gut microbiota

Authors - Shuangfei Ni