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mNeonGreen Polyclonal antibody

mNeonGreen Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29523-1-AP

Print datasheet

Synonyms

blFP-Y3, mNeonGreen

验证数据展示

  • WB analysis using 29523-1-AP

    WB analysis using 29523-1-AP

    mNeonGreen tagged proteins and Native HEK293 cell lysates were subjected to SDS PAGE followed by western blot with 29523-1-AP (mNeonGreen antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

29523-1-AP targets mNeonGreen in WB, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity recombinant protein
Cited Reactivityhuman
Immunogen mNeonGreen fusion protein Ag31617 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mNeonGreen
Synonyms blFP-Y3, mNeonGreen
Calculated Molecular Weight29 kDa
GenBank Accession NumberPF01353
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA0A1S4NYF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

mNeonGreen is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2013, derived from Branchiostoma lanceolatum. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity. This antibody can be used to detect the mNeonGreen tagged recombinant protein.

实验方案

Product Specific Protocols
WB protocol for mNeonGreen antibody 29523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Unlicensed origin DNA melting by MCV and SV40 polyomavirus LT proteins is independent of ATP-dependent helicase activity

Authors - Li Wan
View Article
WB

Elife

A Tad-like apparatus is required for contact-dependent prey killing in predatory social bacteria

Authors - Sofiene Seef
View Article