验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
29523-1-AP targets mNeonGreen in WB, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | human |
| 免疫原 | mNeonGreen fusion protein Ag31617 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mNeonGreen |
| 别名 | mNeon Green, blFP-Y3 |
| 计算分子量 | 29 kDa |
| GenBank蛋白编号 | PF01353 |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_3086140 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A0A1S4NYF2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
mNeonGreen is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2013, derived from Branchiostoma lanceolatum. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity. This antibody can be used to detect the mNeonGreen tagged recombinant protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for mNeonGreen antibody 29523-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Unlicensed origin DNA melting by MCV and SV40 polyomavirus LT proteins is independent of ATP-dependent helicase activity | ||
Elife A Tad-like apparatus is required for contact-dependent prey killing in predatory social bacteria |