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Species Reactivity
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Species Reactivity
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Conjugate
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0
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验证数据展示

  • WB analysis using 28273-1-AP (same clone as 28273-1-PBS)

    WB analysis using 28273-1-AP (same clone as 28273-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28273-1-AP (mTOR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    WB analysis of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    WB result of mTOR antibody (28273-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-mTOR transfected HeLa cells. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    IP experiment of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    IP result of anti-mTOR (IP:28273-1-AP, 4ug; Detection:28273-1-AP 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 28273-1-AP (same clone as 28273-1-PBS)

    IHC staining of human prostate cancer using 28273-1-AP (same clone as 28273-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTOR antibody included
  • IHC staining of human prostate cancer using 28273-1-AP (same clone as 28273-1-PBS)

    IHC staining of human prostate cancer using 28273-1-AP (same clone as 28273-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTOR antibody included
  • IF Staining of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    IF Staining of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using mTOR antibody (28273-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • WB analysis using 28273-1-AP (same clone as 28273-1-PBS)

    WB analysis using 28273-1-AP (same clone as 28273-1-PBS)

    Non-treated and Rapamycin treated lysates were subjected to SDS PAGE followed by western blot with 28881-1-AP (Phospho-mTOR (Ser2448) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (28273-1-AP) and Vinculin (66305-1-Ig) subsequently. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

mTOR Polyclonal antibody, PBS Only

mTOR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 28273-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MTOR Ready-To-Use IHC Kit. MTOR primary antibody included.

产品信息

28273-1-PBS targets mTOR in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 mTOR fusion protein Ag28395 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FK506 binding protein 12-rapamycin associated protein 1
别名
计算分子量 289 kDa
观测分子量 250-289 kDa
GenBank蛋白编号NM_004958
基因名称 mTOR
Gene ID (NCBI) 2475
RRIDAB_2881102
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42345
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).