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  • Featured Product
  • KD/KO Validated

mTOR Polyclonal antibody, PBS Only

mTOR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28273-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 28273-1-AP (same clone as 28273-1-PBS)

    WB analysis using 28273-1-AP (same clone as 28273-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28273-1-AP (mTOR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    WB analysis of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    WB result of mTOR antibody (28273-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-mTOR transfected HeLa cells. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    IP experiment of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    IP result of anti-mTOR (IP:28273-1-AP, 4ug; Detection:28273-1-AP 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 28273-1-AP (same clone as 28273-1-PBS)

    IHC staining of human prostate cancer using 28273-1-AP (same clone as 28273-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTOR antibody included
  • IHC staining of human prostate cancer using 28273-1-AP (same clone as 28273-1-PBS)

    IHC staining of human prostate cancer using 28273-1-AP (same clone as 28273-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTOR antibody included
  • IF Staining of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    IF Staining of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using mTOR antibody (28273-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • WB analysis using 28273-1-AP (same clone as 28273-1-PBS)

    WB analysis using 28273-1-AP (same clone as 28273-1-PBS)

    Non-treated and Rapamycin treated lysates were subjected to SDS PAGE followed by western blot with 28881-1-AP (Phospho-mTOR (Ser2448) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (28273-1-AP) and Vinculin (66305-1-Ig) subsequently. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MTOR Ready-To-Use IHC Kit. MTOR primary antibody included.

产品信息

28273-1-PBS targets mTOR in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen mTOR fusion protein Ag28395 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FK506 binding protein 12-rapamycin associated protein 1
Synonyms
Calculated Molecular Weight 289 kDa
Observed Molecular Weight 250-289 kDa
GenBank Accession NumberNM_004958
Gene Symbol mTOR
Gene ID (NCBI) 2475
RRIDAB_2881102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42345
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).