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Recombinant Mouse Osteopontin protein (His Tag)

种属

Mouse

纯度

>95 %, SDS-PAGE

标签

His Tag

生物活性

EC50: 165-660 ng/mL

Cat no : Eg0317

Print datasheet

Synonyms

Spp1, Opn, Osteopontin

验证数据展示

  • Purity of Recombinant Mouse Osteopontin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Osteopontin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Mouse Osteopontin (His tag) at 0.5 μg/mL (100 μL/well) can bind Human ITGAV (GST tag) with a linear range of 165-660 ng/mL.

    Immobilized Mouse Osteopontin (His tag) at 0.5 μg/mL (100 μL/well) can bind Human ITGAV (GST tag) with a linear range of 165-660 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Mouse Osteopontin (His tag) at 0.5 μg/mL (100 μL/well) can bind Human ITGAV (GST tag) with a linear range of 165-660 ng/mL.
来源 HEK293-derived Mouse Osteopontin protein Leu17-Asn294 (Accession# Q547B5) with a His tag at the C-terminus.
基因ID 20750
蛋白编号 Q547B5
预测分子量 34.5 kDa
SDS-PAGE 50-60 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone. OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues. It can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.

参考文献:

1. Denhardt DT, et al. (2001). J Clin Invest. 107(9):1055-61.
2. Fisher LW, et al. (2001). Biochem Biophys Res Commun. 280(2):460-5.
3. Rittling SR, et al. (2004). Br J Cancer. 90(10):1877-81.
4. Rangaswami H, et al. (2006). Trends Cell Biol. 16(2):79-87.