nNOS Polyclonal antibody

nNOS Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 18984-1-AP

Print datasheet

Synonyms

bNOS, Constitutive NOS, IHPS1, N NOS, NC NOS, Neuronal NOS, Nitric oxide synthase, brain, nNOS, NOS, NOS type I, NOS1



经过测试的应用

Positive IHC detected inmouse heart tissue, mouse kidney tissue, rat testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 3 publications below

产品信息

18984-1-AP targets nNOS in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nitric oxide synthase 1 (neuronal)
Synonyms bNOS, Constitutive NOS, IHPS1, N NOS, NC NOS, Neuronal NOS, Nitric oxide synthase, brain, nNOS, NOS, NOS type I, NOS1
Calculated Molecular Weight 161 kDa
GenBank Accession NumberNM_000620
Gene Symbol nNOS
Gene ID (NCBI) 4842
RRIDAB_10598169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29475
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1).The antibody can recognize isoform 1,2,4 of nNOS.

实验方案

Product Specific Protocols
IHC protocol for nNOS antibody 18984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Mod Pathol

Neuronal distribution in colorectal cancer: associations with clinicopathological parameters and survival

Authors - Maartje Massen
mouse

ACS Chem Neurosci

Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.

Authors - Lei Wu
canineIHC

Exp Eye Res

Altered transsulfuration pathway enzymes and redox homeostasis in inherited retinal degenerative diseases.

Authors - Alireza Badiei
rat

Acta Pharmacol Sin

Neuroprotective effects of scutellarin on rat neuronal damage induced by cerebral ischemia/reperfusion.

Authors - Hu Xia-Min XM
mouse

Biochim Biophys Acta Mol Cell Res

NMDA receptor activation induces damage of alveolar type II cells and lung fibrogenesis through ferroptosis

Authors - Hai-Peng Cheng
pigIHC

Eur J Pharmacol

Suppressive effect of nitric oxide synthase (NOS) inhibitor L-NMMA acetate on choroidal fibrosis in experimental myopic guinea pigs through the nitric oxide signaling pathway

Authors - Tuling Li