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  • Featured Product
  • KD/KO Validated

Nectin-4/PVRL4 Polyclonal antibody

Nectin-4/PVRL4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 21903-1-AP

Print datasheet

Synonyms

Nectin-4, PVRL4, poliovirus receptor related 4, Nectin cell adhesion molecule 4, nectin 4

验证数据展示

  • WB analysis of MCF-7 using 21903-1-AP

    WB analysis of MCF-7 using 21903-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 21903-1-AP

    WB analysis using 21903-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 21903-1-AP

    IHC staining of human placenta using 21903-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Nectin-4/PVRL4 antibody included
  • IHC staining of human placenta using 21903-1-AP

    IHC staining of human placenta using 21903-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Nectin-4/PVRL4 antibody included
  • IF Staining of NIH/3T3 using 21903-1-AP

    IF Staining of NIH/3T3 using 21903-1-AP

    Immunofluorescent analysis of NIH/3T3 cells using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, A2780 cells, HeLa cells, human placenta tissue, NIH/3T3 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy Nectin-4/PVRL4 Ready-To-Use IHC Kit. Nectin-4/PVRL4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21903-1-AP targets Nectin-4/PVRL4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Nectin-4/PVRL4 fusion protein Ag14127 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poliovirus receptor-related 4
Synonyms Nectin-4, PVRL4, poliovirus receptor related 4, Nectin cell adhesion molecule 4, nectin 4
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight 43 kDa, 66 kDa
GenBank Accession NumberBC010423
Gene Symbol Nectin-4
Gene ID (NCBI) 81607
RRIDAB_2878941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).

实验方案

Product Specific Protocols
WB protocol for Nectin-4/PVRL4 antibody 21903-1-APDownload protocol
IHC protocol for Nectin-4/PVRL4 antibody 21903-1-APDownload protocol
IF protocol for Nectin-4/PVRL4 antibody 21903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Oncol

MiR-31 and miR-128 regulates poliovirus receptor-related 4 mediated measles virus infectivity in tumors.

Authors - Hirosha Geekiyanage
View Article
mouseWB,IHC

Int J Urol

Chemotherapy with gemcitabine and cisplatin downregulates tumor expression level of nectin-4 in a syngeneic model of murine MBT2 urothelial cancer cell line and C3H mice

Authors - Makito Miyake
View Article
humanIHC

Oncol Lett

Upregulation of nectin-4 is associated with ITGB1 and vasculogenic mimicry and may serve as a predictor of poor prognosis in colorectal cancer.

Authors - Jinxiu Zhang
View Article
human,mouseWB

Cell Biosci

Interferon-stimulated gene PVRL4 broadly suppresses viral entry by inhibiting viral-cellular membrane fusion

Authors - Qiaomei Cai
  • KD Validated
View Article