验证数据展示
经过测试的应用
Positive WB detected in | SGC-7901 cells, U-251 cells, U-87 MG cells |
Positive IF-P detected in | rat brain tissue, human kidney tissue |
Positive IF/ICC detected in | U-251 cells |
Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 2 publications below |
产品信息
29285-1-AP targets Nestin in WB, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, IF-P, FC (Intra), ELISA Application Description |
文献引用应用 | IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | Nestin fusion protein Ag29865 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | nestin |
别名 | NES |
计算分子量 | 177 kDa |
观测分子量 | 240-280 kDa |
GenBank蛋白编号 | NM_006617 |
基因名称 | Nestin |
Gene ID (NCBI) | 10763 |
RRID | AB_2918268 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P48681 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).
实验方案
Product Specific Protocols | |
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WB protocol for Nestin antibody 29285-1-AP | Download protocol |
IF protocol for Nestin antibody 29285-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Bioact Mater Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair | ||
Brain Behav Immun Microbial reconstitution reverses prenatal stress-induced cognitive impairment and synaptic deficits in rat offspring |