• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 31221-1-AP

    WB analysis using 31221-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 31221-1-AP (P14-ARF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 31221-1-AP

    IHC staining of human ovary cancer using 31221-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31221-1-AP (P14-ARF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDKN2A/P16INK4A antibody included
  • IF Staining of HeLa using 31221-1-AP

    IF Staining of HeLa using 31221-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using P14-ARF antibody (31221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

P14-ARF Polyclonal antibody

P14-ARF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31221-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Alternative reading frame ARF, p14ARF, p19ARF, CDKN2A, MTS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDKN2A/P16INK4A Ready-To-Use IHC Kit. CDKN2A/P16INK4A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31221-1-AP targets P14-ARF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 P14-ARF fusion protein Ag33340 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 2A
别名 Alternative reading frame ARF, p14ARF, p19ARF, CDKN2A, MTS1
计算分子量14kd
观测分子量 19 kDa
GenBank蛋白编号NM_058195
基因名称 CDKN2A
Gene ID (NCBI) 1029
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N726
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDKN2A locus on chromosome 9p21 encodes two transcripts/proteins INK4A and ARF. INK4A (also referred to as p16INK4A) specially blocks the CDK4 and CDK6 cyclin-dependent kinases that control the phosphorylation status of the retinoblastoma (RB) protein (PMID: 11584300, PMID: 8259215). ARF (known as p14ARF in human and p19ARF in mouse) has been designed as a positive regulator of p53 levels because, through its complexation with Mdm2 (HDM2 in human), it prevents mdm2-mediated cytoplasmic translocation and degradation of p53 (PMID: 9153396).

实验方案

Product Specific Protocols
WB protocol for P14-ARF antibody 31221-1-APDownload protocol
IHC protocol for P14-ARF antibody 31221-1-APDownload protocol
IF protocol for P14-ARF antibody 31221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols