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p300 Polyclonal antibody, PBS Only

p300 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20695-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 20695-1-AP (same clone as 20695-1-PBS)

    WB analysis using 20695-1-AP (same clone as 20695-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 20695-1-AP (same clone as 20695-1-PBS)

    IHC staining of mouse lung using 20695-1-AP (same clone as 20695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EP300 antibody included
  • IHC staining of mouse lung using 20695-1-AP (same clone as 20695-1-PBS)

    IHC staining of mouse lung using 20695-1-AP (same clone as 20695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EP300 antibody included
  • IHC staining of rat lung using 20695-1-AP (same clone as 20695-1-PBS)

    IHC staining of rat lung using 20695-1-AP (same clone as 20695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EP300 antibody included

It is recommended to use NETN lysis buffer for sample preparation during WB detection.

Planning an IHC experiment? We recommend our IHCeasy EP300 Ready-To-Use IHC Kit. EP300 primary antibody included.

产品信息

20695-1-PBS targets p300 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name E1A binding protein p300
Synonyms
Calculated Molecular Weight 264 kDa
Observed Molecular Weight300 kDa
GenBank Accession NumberNM_001429
Gene Symbol EP300
Gene ID (NCBI) 2033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ09472
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).