验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | THP-1 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83078-4-RR targets p300 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Affinity | KD=1.30 x 10-8M KOff=3.14 x 10-3M KOn=2.42 x 105M |
Full Name | E1A binding protein p300 |
Synonyms | EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300 |
Calculated Molecular Weight | 264 kDa |
GenBank Accession Number | NM_001429 |
Gene Symbol | EP300 |
Gene ID (NCBI) | 2033 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q09472 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).
实验方案
Product Specific Protocols | |
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IF protocol for p300 antibody 83078-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |