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验证数据展示

  • FC experiment of THP-1 using 83078-4-RR

    FC experiment of THP-1 using 83078-4-RR

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug p300 Recombinant antibody (83078-4-RR, Clone:230369H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of HepG2 using 83078-4-RR

    IF Staining of HepG2 using 83078-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-4-RR, Clone: 230369H8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

p300 Recombinant antibody

p300 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83078-4-RR

产品说明书

CloneNo. 230369H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy EP300 Ready-To-Use IHC Kit. EP300 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83078-4-RR targets p300 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
亲和力
KD=1.30 x 10-8M
KOff=3.14 x 10-3M
KOn=2.42 x 105M
全称 E1A binding protein p300
别名 EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300
计算分子量 264 kDa
GenBank蛋白编号NM_001429
基因名称 EP300
Gene ID (NCBI) 2033
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ09472
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).

实验方案

Product Specific Protocols
IF protocol for p300 antibody 83078-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols