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Species Reactivity
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验证数据展示

  • FC experiment of THP-1 using 83078-4-RR

    FC experiment of THP-1 using 83078-4-RR

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug p300 Recombinant antibody (83078-4-RR, Clone:230369H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of HepG2 using 83078-4-RR

    IF Staining of HepG2 using 83078-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-4-RR, Clone: 230369H8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

p300 Recombinant antibody

p300 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83078-4-RR
CloneNo. 230369H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy EP300 Ready-To-Use IHC Kit. EP300 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83078-4-RR targets p300 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=1.30 x 10-8M
KOff=3.14 x 10-3M
KOn=2.42 x 105M
Full Name E1A binding protein p300
Synonyms EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300
Calculated Molecular Weight 264 kDa
GenBank Accession NumberNM_001429
Gene Symbol EP300
Gene ID (NCBI) 2033
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ09472
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).

实验方案

Product Specific Protocols
IF protocol for p300 antibody 83078-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols