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p38 MAPK Polyclonal antibody

p38 MAPK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30382-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 30382-1-AP

    WB analysis using 30382-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30382-1-AP (p38 MAPK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 30382-1-AP

    IF Staining of HepG2 using 30382-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using p38 MAPK antibody (30382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, PC-12 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy p38 MAPK Ready-To-Use IHC Kit. p38 MAPK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30382-1-AP targets p38 MAPK in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen p38 MAPK fusion protein Ag33174 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 14
Synonyms
Calculated Molecular Weight 360 aa, 41 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC031574
Gene Symbol p38 MAPK
Gene ID (NCBI) 1432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16539
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for p38 MAPK antibody 30382-1-APDownload protocol
IF protocol for p38 MAPK antibody 30382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols