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p504S,AMACR Monoclonal antibody, PBS Only

p504S,AMACR Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H9B5

Cat no : 60240-1-PBS

Print datasheet

Synonyms

AMACR, p504S, EC:5.1.99.4, AMACR/p504S, Alpha-methylacyl-CoA racemase

验证数据展示

  • WB analysis using 60240-1-Ig (same clone as 60240-1-PBS)

    WB analysis using 60240-1-Ig (same clone as 60240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

  • WB analysis using 60240-1-Ig (same clone as 60240-1-PBS)

    WB analysis using 60240-1-Ig (same clone as 60240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 60240-1-Ig (same clone as 60240-1-PBS)

    IF Staining of A431 using 60240-1-Ig (same clone as 60240-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using p504S,AMACR antibody (60240-1-Ig, Clone: 2H9B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

产品信息

60240-1-PBS targets p504S,AMACR in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen p504S,AMACR fusion protein Ag8720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alpha-methylacyl-CoA racemase
Synonyms AMACR, p504S, EC:5.1.99.4, AMACR/p504S, Alpha-methylacyl-CoA racemase
Calculated Molecular Weight 382 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC009471
Gene Symbol AMACR
Gene ID (NCBI) 23600
RRIDAB_2881363
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UHK6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).