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验证数据展示

  • WB analysis using 60202-1-Ig (same clone as 60202-1-PBS)

    WB analysis using 60202-1-Ig (same clone as 60202-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 60202-1-Ig (same clone as 60202-1-PBS)

    WB analysis of HepG2 using 60202-1-Ig (same clone as 60202-1-PBS)

    WB result of peroxiredoxin 2 antibody (60202-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

    IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

    IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 60202-1-Ig (same clone as 60202-1-PBS)

    IF Staining of MCF-7 using 60202-1-Ig (same clone as 60202-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using peroxiredoxin 2 antibody (60202-1-Ig, Clone: 3H6C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

peroxiredoxin 2 Monoclonal antibody, PBS Only

peroxiredoxin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60202-1-PBS

产品说明书

CloneNo. 3H6C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60202-1-PBS targets peroxiredoxin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 peroxiredoxin 2 fusion protein Ag0835 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxiredoxin 2
别名 PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC003022
基因名称 peroxiredoxin 2
Gene ID (NCBI) 7001
RRIDAB_10918978
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP32119
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)