Phospho-TDP43 (Ser409/410) Monoclonal antibody

Phospho-TDP43 (Ser409/410) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1A2C1

Cat no : 66318-1-Ig

Print datasheet

Synonyms

ALS10, phospho(409/410)-TDP43, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43



经过测试的应用

Positive WB detected inEA treated HeLa cells, HEK-293 cells, human placenta tissue, Neuro-2a cells, C6 cells
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66318-1-Ig targets Phospho-TDP43 (Ser409/410) in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TAR DNA binding protein
Synonyms ALS10, phospho(409/410)-TDP43, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 45-50 kDa, 35 kDa
GenBank Accession NumberNM_007375
Gene Symbol TDP-43
Gene ID (NCBI) 23435
RRIDAB_2881699
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene.In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 66318-1-Ig is a phospho-dependent monoclonal antibody specifically recognizing phospho-TDP43 (409/410). It does not react with native TDP-43.

实验方案

Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser409/410) antibody 66318-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Mol Cell

Stress Induces Dynamic, Cytotoxicity-Antagonizing TDP-43 Nuclear Bodies via Paraspeckle LncRNA NEAT1-Mediated Liquid-Liquid Phase Separation.

Authors - Chen Wang
humanIHC

Acta Neuropathol Commun

Ex vivo MRI atlas of the human medial temporal lobe: characterizing neurodegeneration due to tau pathology.

Authors - Sadhana Ravikumar

PLoS Genet

Progressive ataxia of Charolais cattle highlights a role of KIF1C in sustainable myelination.

Authors - Amandine Duchesne
humanIHC

Eur J Neurol

SOD1 p.D12Y variant is associated with amyotrophic lateral sclerosis/distal myopathy spectrum.

Authors - G Tasca
humanIHC

Neuroimage Clin

Ex vivo MRI and histopathology detect novel iron-rich cortical inflammation in frontotemporal lobar degeneration with tau versus TDP-43 pathology.

Authors - M Dylan Tisdall
humanWB,IF

Mol Cell Biol

The N-termini of TAR DNA-binding protein-43 (TDP43) C-terminal fragments influence degradation, aggregation propensity and morphology.

Authors - Yasar Arfat T Kasu